刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺

邵佳锋; 刘树民; 牟洪; 范振群; 安丽凤; 董杨

doi:10.13422/j.cnki.syfjx.2011.02.013

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刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺

工艺与制剂 | 更新时间：2024-09-23

- 刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺
- Separation and Purification of Syringin and Elentheroside E by Macroporous Adsorbents
- 中国实验方剂学杂志 2011年17卷第2期页码：10-14
- 作者机构：
  
  黑龙江中医药大学中医药研究院
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项（2009ZX09103）
- DOI：10.13422/j.cnki.syfjx.2011.02.013
  中图分类号： R284
- 纸质出版日期：2011
- 稿件说明：
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[1]邵佳锋,刘树民,牟洪,范振群,安丽凤,董杨.刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺[J].中国实验方剂学杂志,2011,17(02):10-14.

SHAO Jia-feng, LIU Shu-min, MOU Hong, et al. Separation and Purification of Syringin and Elentheroside E by Macroporous Adsorbents[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 10-14.
[1]邵佳锋,刘树民,牟洪,范振群,安丽凤,董杨.刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺[J].中国实验方剂学杂志,2011,17(02):10-14. DOI： 10.13422/j.cnki.syfjx.2011.02.013.

SHAO Jia-feng, LIU Shu-min, MOU Hong, et al. Separation and Purification of Syringin and Elentheroside E by Macroporous Adsorbents[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 10-14. DOI： 10.13422/j.cnki.syfjx.2011.02.013.

摘要

目的:研究大孔树脂分离纯化紫丁香苷、刺五加苷E的工艺。方法:采用AB-8型大孔吸附树脂对紫丁香苷、刺五加苷E进行吸附纯化

并采用HPLC

以紫丁香苷、刺五加苷E含量和收率为参考指标综合评价。结果:优选纯化工艺为

上样液的质量浓度0.5 g·mL-1

吸附流速2 BV·h-1

洗脱剂30%乙醇

用量9 BV

洗脱流速1 BV·h-1。结论:刺五加提取物通过AB-8树脂

30%乙醇洗脱部位

可使紫丁香苷、刺五加苷E的收率和质量分数达到满意效果。

Abstract

Objective:To establish a technical process for the purification of syringin and eleutheroside E from Acanthopanax senticosus.Method:AB-8 type of macroporous adsorbents was used to separate and purify syringin and eleutheroside E

and HPLC was adopted to analyze the content and yield of syringin and eleutheroside E.Result:The optimum process for the purification was as follows:the concentration of sample solution was 0.5 g·mL-1;adsorption flow rate was 2 BV·h-1;eluent was 30% ethanol;the dosage was 9 BV;elution flow rate was 1 BV·h-1.Conclusion:Extract of A.senticosus by AB-8 resin

30% ethanol elution positions can syringin

eleutheroside E

yield and quality of the scores achieved satisfactory results.

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