CHEN Zhan-ke1, WANG Shu-mei2, GAO Shan1, et al. Study on Deproteinization of Polysaccharide from Polygoni Multiflori Radix Preparata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 41-43.
CHEN Zhan-ke1, WANG Shu-mei2, GAO Shan1, et al. Study on Deproteinization of Polysaccharide from Polygoni Multiflori Radix Preparata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 41-43. DOI: 10.13422/j.cnki.syfjx.2011.04.003.
Objective:To screen out the the optimal technology for deproteinization of polysaccharide from Radix Polygoni Multiflori Preparata.Method:The protein was removed by the enzyme
TCA and Sevage methods with the percentage of the removed protein(the PRP) and the decomposed polysaccharide(the PDP) as index.Result: The PRPs for the enzyme
TCA and Sevage methods were 87.62%
73.12% and 62.56%
the PDPs were 8.13%
29.36% and 20.24%.Conclusion:Enzymatic method of removing protein is an effective and alternative method.