何首乌二苯乙烯苷大孔树脂纯化工艺优化

陈伟; 李霞; 郑友兰; 孙连娜

doi:10.13422/j.cnki.syfjx.2011.05.002

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何首乌二苯乙烯苷大孔树脂纯化工艺优化

工艺与制剂 | 更新时间：2024-09-23

- 何首乌二苯乙烯苷大孔树脂纯化工艺优化
- Optimization of Tetrahydroxystilbene Glucoside Separation from Radix Poligoni Multiflori by Macro-reticular Resin ZTC-1
- 中国实验方剂学杂志 2011年17卷第5期页码：40-43
- 作者机构：
  
  1. 吉林农业大学中药材学院
  2. 第二军医大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.002
  中图分类号： TQ461
- 纸质出版日期：2011
- 稿件说明：
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[1]陈伟,李霞,郑友兰,孙连娜.何首乌二苯乙烯苷大孔树脂纯化工艺优化[J].中国实验方剂学杂志,2011,17(05):40-43.

CHEN Wei1, LI Xia2, ZHENG You-lan1, et al. Optimization of Tetrahydroxystilbene Glucoside Separation from Radix Poligoni Multiflori by Macro-reticular Resin ZTC-1[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 40-43.
[1]陈伟,李霞,郑友兰,孙连娜.何首乌二苯乙烯苷大孔树脂纯化工艺优化[J].中国实验方剂学杂志,2011,17(05):40-43. DOI： 10.13422/j.cnki.syfjx.2011.05.002.

CHEN Wei1, LI Xia2, ZHENG You-lan1, et al. Optimization of Tetrahydroxystilbene Glucoside Separation from Radix Poligoni Multiflori by Macro-reticular Resin ZTC-1[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 40-43. DOI： 10.13422/j.cnki.syfjx.2011.05.002.

摘要

目的:筛选ZTC-1树脂分离纯化何首乌二苯乙烯苷的最佳工艺条件。方法:高效液相色谱法测定二苯乙烯苷的含量

考察ZTC-1树脂的吸附性能及最佳洗脱条件

并通过重复验证试验及中试试验确定合理的工艺。结果:ZTC-1树脂对二苯乙烯苷的的最佳分离工艺为

1倍药材量等体积上样

依次用5 BV蒸馏水、10 BV的30%乙醇、10 BV的95%乙醇梯度洗脱

流速为2.5 BV.h-1

并对30%乙醇部位回收溶剂后依上述流程纯化。结论:ZTC-1大孔吸附树脂可用于何首乌二苯乙烯苷的精制。

Abstract

Objective:To evaluate the performance of macro-reticular resin ZTC-1 for the separation and purification of tetrahydroxystilbene glucoside from Radix Poligoni Multiflori.Method:The concentration of tetra-hydroxystilbene glucoside was determined by HPLC.The optimum process conditions were obtained through the study of the dynamic adsorption and desorption characteristics of macroporous adsorption resins by small scale and pilot scale tests.Result:The adsorption and elution characteristics of macro-reticular resin ZTC-1 used to separate and purify tetrahydroxystilbene glucoside were very good.In the course of adsorption

the amount of used adsorption was 1 g dried mL-1 resin and the volumetric rate was 2.5 BV.h-1;in the course of elution

the resin column chromatogra-phy was eluted gradient with 10 BV 30% and 10 BV 70% EtOH after removing impurities with 5 BV water

and the 30% ethanol fractions were required to be purified again according to the above process after recovering the solvent.Conclusion:Macro-reticular resin ZTC-1 can be used to refine the tetrahydroxystilbene glucoside from the extraction of Radix Poligoni Multiflori.

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