SUN Jing1, XUE Rui3, LIU Jie-qiong1. Research on Improvement of Propolis Extracting Technology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 33-36.
SUN Jing1, XUE Rui3, LIU Jie-qiong1. Research on Improvement of Propolis Extracting Technology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 33-36. DOI: 10.13422/j.cnki.syfjx.2011.05.039.
Objective:To research the changes of flavonoids content and the rate of extractum extracted be-tween improvement technology and traditional technology
extraction technique to be improved.Method:Rutin was used as reference substance to determine the total flavonoid content in propolis by UV spectrophotometry.three dif-ferent extraction methods including soaking method
ultrasonic extraction
cold soak and the extraction temperature were investigated with total flavonoids and the rate of extractum extracted as the investigation indexes;4 factors of ethanol concentration
extraction time and ethanol
extraction times were investigated by orthogonal design.Parallel testing was adopted in the investigations.Result:Extraction times had significant impact on the total flavonoids of propolis at 60 ℃.The optimum technology conditions were as follows:10 times amount of 75% ethanol
soaking 3 times
extracted for 2 hours each time.The total flavonoids increased 14.43% and the rate of extractum extracted decreased 61.71%.Conclusion:Propolis wax removed before the effective parts of the extraction could greatly reduce the cream and did not decrease the effective site content
better than the effective parts of traditional direct extraction process and the method is simpler.