ZHOU Nong, DUAN Bao-zhong, XIA Cong-long, et al. Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 70-73.
ZHOU Nong, DUAN Bao-zhong, XIA Cong-long, et al. Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 70-73. DOI: 10.13422/j.cnki.syfjx.2011.05.046.
滇重楼不同生长发育期化学成分的变化规律
摘要
目的:建立滇重楼HPLC指纹图谱
考察滇重楼不同生长期主要化学成分的变化。方法:采用高效液相色谱法。色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm
Objective:To establish HPLC chromatographic fingerprint of Paris polyphylla var.yunnanensis and investigate variation of the main components in samples at various collecting seasons.Method:The main com-ponents were separated on a Agela Venusil XBP-C18(4.6 mm × 250 mm
5 μm) column and detected at 210 nm by gradient elution at the flow rate of 1.0 mL·min-1 using acetonitrile-water as the mobile phase.Column temperature was at 35 ℃.Result:A satisfactory method for HPLC fingerprint determination of P.polyphylla var.yunnanensis was obtained.The established fingerprint showed that 20 peaks were common and 4 peaks were identified.The main com-ponents of P.polyphylla var.yunnanensis differed from collecting seasons.Conclusion:The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of P.polyphylla var.yunnanensis.