CHEN Tian-chao1, ZHAI Lai-chao2. HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 105-107.
CHEN Tian-chao1, ZHAI Lai-chao2. HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 105-107. DOI: 10.13422/j.cnki.syfjx.2011.05.056.
HPLC同时测定地黄中梓醇与毛蕊花糖苷的含量
摘要
目的:研究地黄中梓醇与毛蕊花糖苷的含量测定方法
为其质量评价提供依据。方法:采用HPLC梯度洗脱法
在同一色谱条件下测定含量。色谱条件:AgilentSB-AQ色谱柱(4.6 mm×250 mm
5μm)
流速1 mL·min-1
柱温25℃
检侧波长210 nm。结果:两种成分均能达到基线分离
线性良好。结论:经过系统的方法学考察
该方法操作简便、结果准确、重现性好
适用于地黄2010年版药典规定的这2种苷类成分的含量测定。
Abstract
Objective:To study the determination method about the catalpol and verbascoside
provide basis for quality evaluation.Method:Gradient elution was applied in HPLC analysis.Agilent SB-AQ chromatographic column(4.6 mm × 250 mm
5 μm)
velocity 1mL·min-1
column temperature was 25 ℃
wavelength was 210 nm.Result:Two components can achieve good baseline separation
linear.Conclusion:After the systematic methodology
the method is simple
accurate
reproducible
applicable to the provisions by pharmacopoeia rehmannia glutinosa two content determination.