Objective:To compare the anti-depressant effects of Guizhi Jieyu Fang by different techniques.Method:Mice were divided randomly into four groups:two treatment groups（Guizhi Jieyu Fang-A and Guizhi Jieyu Fang-B orally given for 10 days

12.66 g.kg-1

daily）

amitriptyline hydrochloride group（amitriptyline hydrochlo-ride orally given for 3 days 50 g.kg-1

daily）and control group.The forced swimming test

tail suspension test

open field activity test and reserpine-induced hypothermia test were carried out to determine the antidepressant activity in mice.Mice immobility time in forced swimming test and tail suspension test

spontaneous activity frequency in open field activity test

and body temperature decrease in reserpine-induced hypothermia test were observed.Result:Guizhi Jieyu Fang-A could significantly shorten immobility time in forced swimming test and tail suspension test and inhabit the decrease of body temperature in reserpine antagonistic test at the 4th hour（P < 0.05）

but no impact in open field activity test.Conclusion:Guizhi Jieyu Fang-A is better than Guizhi Jieyu Fang-B for anti-depression.