Objective:To study the hepato-protective effect and the related mechanisms of protoporphyrin disodium（NAPP） on carbon tetrachloride（CCl4） induced acute hepatic injury in mice.Method:Sixty ICR mice were randomly divided into normal control group

model group

bifendate pills group

NAPP low dose group

NAPP medium dose group and NAPP high dose group

with 10 animals in each group.Bifendate pills（0.2 g.kg-1） or dif-ferent dosages（0.03

0.06

0.12 g.kg-1）of NAPP were given for each treatment by intragastric administration con-tinuously for 10 days and then a single dose of CCl4 at 0.06 g.kg-1 CCl4was given by intraperitoneal injection to es-tablish acute hepatic injury model.Sixteen hours later

blood was collected to detect the activities of alanine amin-otransferase（ALT）and aspartate aminotransferase（AST）in serum·Liver was taken out by laparotomy and weighed to calculate liver index

and liver homogenate was prepared to determine the activities of catalase（CAT）

glutathione peroxidase（GSH-Px）and superoxide dismutase（SOD） and the content of malondialdehyde（MDA）.The observationof liver histopathological change was made for the mice.Result:Compared with CCl4model group

the liver indices and the activities of ALT and AST in serum of each treatment group were decreased significantly to various degrees（P < 0.05

P < 0.01）

the activities of CAT

GSH-Px and SOD in liver homogenate of each treatment group were in-creased dramatically at different extent（P < 0.05

P < 0.01）

and the content of MDA of each treatment group in liver homogenate was reduced remarkably at different degree（P < 0.05

P < 0.01）.The pathological sections showed that hepatic injury of each treatment group was relieved to different extent clearly

and the slightest group was NAPP high dose group.Conclusion:NAPP has a good protective effect on the acute hepatic injury in mice induced by CCl4

and the mechanism is probably related with the anti-lipid-peroxidation effect and the prevention of the decrease of liver anti-oxidative enzyme activities.