DENG Chong, YAN Yong-gang. Multi-index Evaluation of Extraction of Phenolic Compounds from Sargentodoxa cuneata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 12-14.
DENG Chong, YAN Yong-gang. Multi-index Evaluation of Extraction of Phenolic Compounds from Sargentodoxa cuneata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 12-14. DOI: 10.13422/j.cnki.syfjx.2011.13.022.
Objective:To screen out the best extraction technique of chemical composition of phenolic acids from cuneata.Method: The HPLC method for determination of gallic acid
protocatechuic acid and chlorogenic acid in Sargentodoxa cuneata was firstly established.The orthogonal experimental design was used to study the optimum extraction with extracting time
ethanol concentration
solvent usage
extraction times as factors
gallic acid
protocatechuic acid
chlorogenic acid as the evaluation index to determine the best extraction technology.Result: the optimum extraction technology was 80% ethanol concentration
10 times of solvent usage
extracted for 30min
3 times of extraction.Conclusion: A multi-index evaluation method can optimize the extraction process conditions of chemical composition of phenolic acids from S.cuneata and provide a reference for separation and purification of total phenolic acids from S.cuneata.