Objective:To investigate the association of the pathogenesis and drug action target of Jingqianping and Jingqianshu granule with the expression level of 5-HTR2C in hypothalamus of rats with anger-in and anger-out constitution.Method:The model rats were prepared by the methods of residential intrusion

and treated with Jingqianping or Jingqianshu granule.Then we gained control groups

model group and treated groups.The expression of 5-HTR2C in the hypothalamus of the models was detected by RT-PCR and western blotting.Result:The levels of 5-HTR2C mRNA and protein in the hypothalamus of models decreased significantly as compared to the controls

and the levels of the anger-out-model group were higher than the anger-in group.And the levels of 5-HTR2C mRNA and protein in groups treated with Jingqianping or Jingqianshu granule increased significant as compared to the models.There were significantly difference for mRNA between the treated and the control rats.There were no significant difference for protein between the treated and the control rats.Conclusion:The expression of 5-HTR2C in the hypothalamus of the two model group decreased significantly compared to the controls

and then the protein recovered to the control level and the mRNA was adjusted after treated with Jingqianping or Jingqianshu granule.So

the result demonstrates that the association of the expression of 5-HTR2C maybe exist with anger-out and anger-in.And the gene could be one of the action target of Jingqianping and Jingqianshu granule.