TAN Feng, FAN Qiao-ling, WANG Ming-yan, et al. Influence of Zuogui Wan on Proliferation of Bone Mesenchymal Stem Cells of SD Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 145-148.
TAN Feng, FAN Qiao-ling, WANG Ming-yan, et al. Influence of Zuogui Wan on Proliferation of Bone Mesenchymal Stem Cells of SD Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 145-148. DOI: 10.13422/j.cnki.syfjx.2011.13.060.
Objective:To observe the influences of water extract of Zuogui Wan on the proliferation of bone mesenchymal stem cells(BMSCs) of SD rats in vitro.Method: BMSCs of SD rats were isolated with adherent separation method and primarily cultured in vitro
morphologically observed under inverted microscope
and identified with immunohistochemical method.The proliferation of BMSCs effected by different concentration of water extract of Zuogui Wan were detected with MTT assay.Result: The BMSCs presented a long spindle shape and a colony growth pattern.CD44
CD29 on BMSCs were positive and CD34 was negative detected by immunohistochemical method.MTT assay showed absorbance of BMSCs treated with Zuogui Wan for 24 hours at the dose range of 2.5-4 g·L-1(crude drug) was significantly higher than that of control group(P<0.01)
especially the absorbance at 1 g·L-1.Conclusion: The BMSCs isolated with adherent separation method in this experiment presented the characteristics of BMSCs both in phenotypic expression and cell morphology.Within certain range of concentrations
water extract of Zuogui Wan could improve the proliferation activity of BMSCs in vitro.