桃仁对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响

李小波; 边壮; 兰萍; 刘颖

doi:10.13422/j.cnki.syfjx.2011.13.072

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桃仁对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响

药理 | 更新时间：2024-09-23

- 桃仁对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响
- Influnence of Persicae Semen on Epithelial-mesenchymal Transdifferentiation of Renal Tubular in Rats with Unilateral Ureteral Obstruction
- 中国实验方剂学杂志 2011年17卷第13期页码：189-191
- 作者机构：
  
  1. 遵义医学院珠海校区临床技能中心诊断学实验室
  2. 中山大学附属第五医院
- 作者简介：
- 基金信息：
  
  贵州省科技厅课题（黔科合丁字LKZ[2010]37号）
- DOI：10.13422/j.cnki.syfjx.2011.13.072
  中图分类号： R285.5
- 纸质出版日期：2011
- 稿件说明：
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[1]李小波,边壮,兰萍,刘颖.桃仁对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响[J].中国实验方剂学杂志,2011,17(13):189-191.

LI Xiao-bo1, BIAN Zhuang2, LAN Ping2, et al. Influnence of Persicae Semen on Epithelial-mesenchymal Transdifferentiation of Renal Tubular in Rats with Unilateral Ureteral Obstruction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 189-191.
[1]李小波,边壮,兰萍,刘颖.桃仁对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响[J].中国实验方剂学杂志,2011,17(13):189-191. DOI： 10.13422/j.cnki.syfjx.2011.13.072.

LI Xiao-bo1, BIAN Zhuang2, LAN Ping2, et al. Influnence of Persicae Semen on Epithelial-mesenchymal Transdifferentiation of Renal Tubular in Rats with Unilateral Ureteral Obstruction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 189-191. DOI： 10.13422/j.cnki.syfjx.2011.13.072.

摘要

目的:探讨桃仁对单侧输尿管结扎诱导的肾间质纤维化实验大鼠肾小管上皮细胞转分化的影响。方法:采用单侧输尿管结扎（UUO）肾间质纤维化模型。将大鼠随机分为4组

正常组、模型组ig给2 mL自来水

蒙诺组ig予10 mg·kg-1和桃仁组ig予6 g·kg-1释成冲剂2 mL

每日1次。术后14 d处死大鼠

观察肾组织病理改变

并应用免疫组织化学方法检测肾组织上皮细胞钙黏蛋白（E-cadherin）

α-平滑肌激动蛋白（α-SMA）和纤维连接蛋白（FN）的表达。结果:与正常组相比

模型组肾小管上皮细胞E-cadherin有较弱表达

肾间质α-SMA和FN有较强的表达（P<0.05）;与模型组相比

桃仁组纤维化程度减轻

且α-SMA和FN的阳性表达较模型组显著减弱

E-cadherin表达较强

有统计学差异（P<0.05）

桃仁组与蒙诺组比无统计学差异少。结论:桃仁可能通过增强E-cadherin、减弱α-SMA和FN的表达改善肾小管上皮细胞转分化

而减缓肾间质纤维化病程进展。

Abstract

Objective:To observe the influence of Persicae Semen on tubular erithelial myofibroblast transdifferentiation in rats with renal interstitial fibrosis induced by unilateral ureteral obstruction.Method: Rat model of unilateral ureteral obstruction was used.The rats were randomly divided into 4 groups:nomal group

mode group

monopril treatment group

Persicae Semen treatment group.Renal tissue was examined by light microscopy at the 2th week after operation.Immuno-histochemistry was applied to measure the expression of E-cadherin

α-SMA and FN in the renal interstitium.Result:Fibrosis was observed in the renal interstitium of rats with obstructive nephropathy

the expression of α-SMA and FN in the normal operation group was low in the renal tubular epithellal cell and renal interstitium cell

but the expression of E-cadherin was high.In comparison with normal group

the expression of α-SMA and FN of model group was markedly increased

but the expression of E-cadherin was decreased（P<0.05）.In comparison with model group

Persicae Semen group could significantly decrease the expression of α-SMA and FN

but the expression of E-cadherin was increased（P<0.05）;no significant difference was detected between Persicae Semen treatment group and monopril treatment group.Conclusion: Pesicae semen may suppress fibrosis development

at least in part via down regulating α-SMA and FN expression.

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