ZHAN Xue-kong1, ZHOU Hai-mei1, MA Xiao-hua2, et al. New Extraction Technology of Polysaccharide from Sea Cucumber[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 40-42.
ZHAN Xue-kong1, ZHOU Hai-mei1, MA Xiao-hua2, et al. New Extraction Technology of Polysaccharide from Sea Cucumber[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 40-42. DOI: 10.13422/j.cnki.syfjx.2011.15.001.
海参多糖提取新工艺
摘要
目的:优选海参多糖的最佳提取工艺。方法:采用正交试验法
对提取海参多糖的影响因素进行研究
并以其含量为评价指标。结果:海参多糖的最佳提取工艺为以2倍体积的3%NaOH溶液进行提取后
再以2%的胰蛋白酶(酶解条件:pH 8.0
温度40℃
2 h)及2%的胃蛋白酶(酶解条件:pH 4.0
温度40℃
2 h)进行双酶水解
测得海参多糖质量分数达2.51%。结论:该提取工艺方法简单易行
重复性好
具可行性
可用于海参多糖的提取。
Abstract
Objective:To optimize the best extraction of sea cucumber polysaccharide.Method:Orthogonal test was applied to study the sea cucumber polysaccharide extracted factors with its content as evaluation index.Result:The best extraction of sea cucumber polysaccharide was as follows:2 times the volume of 3% NaOH solution was extracted
and then 2% trypsin(hydrolysis conditions:pH 8.0
temperature 40 ℃
time 2 h) and 2% pepsin(hydrolysis conditions:pH 4.0
temperature 40 ℃
time 2 h) were used for dual enzymatic hydrolysis;the content of sea cucumber polysaccharide measured was 2.51%.Conclusion:The extraction method is simple
reproducible and feasible;it can be used for sea cucumber polysaccharide extraction.