REN Shou-li1, LIU Ta-shi1, LIN Li-mei1, et al. Determination of Gastrodin and P-Hydroxybenzyl Alcohol in Different Commercial Grades of Gastrodia elata by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 55-58.
REN Shou-li1, LIU Ta-shi1, LIN Li-mei1, et al. Determination of Gastrodin and P-Hydroxybenzyl Alcohol in Different Commercial Grades of Gastrodia elata by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 55-58. DOI: 10.13422/j.cnki.syfjx.2011.15.028.
Objective:To establish the determination of gastrodin and p-hydroxybenzyl alcohol in Gastrodia elata
and determine the different commercial grades of Gastrodia elata.Method:The samples were separated at 25℃ on ZORBAX Eclipse XDB-C18 column eluted with 0.1% phosphoric acid-methanol(95:5) as mobile phase
flowrate was 1 mL.min-1 and detection wavelength was set at 220 nm.Result:The sample quality of gastrodin and p-hydroxybenzyl alcohol with the peak area showed good linear relationship
the average recoveries were 100.78%(n=6) and 101.59%(n=6)
the content of the different origin and products specifications was significant difference.Conclusion:The method is simple
accurate and reproducibility
it can provide the basis of rational application
quality control and the standards for Gastrodia elata.