HPLC同时测定安渴胶囊中马钱苷和葛根素的含量

牛晓静; 施钧瀚

doi:10.13422/j.cnki.syfjx.2011.15.038

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HPLC同时测定安渴胶囊中马钱苷和葛根素的含量

化学与分析 | 更新时间：2024-09-23

- HPLC同时测定安渴胶囊中马钱苷和葛根素的含量
- Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC
- 中国实验方剂学杂志 2011年17卷第15期页码：90-92
- 作者机构：
  
  河南中医学院第一附属医院药学部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.038
  中图分类号： R286.0
- 纸质出版日期：2011
- 稿件说明：
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[1]牛晓静,施钧瀚.HPLC同时测定安渴胶囊中马钱苷和葛根素的含量[J].中国实验方剂学杂志,2011,17(15):90-92.

NIU Xiao-jing, SHI Jun-han. Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 90-92.
[1]牛晓静,施钧瀚.HPLC同时测定安渴胶囊中马钱苷和葛根素的含量[J].中国实验方剂学杂志,2011,17(15):90-92. DOI： 10.13422/j.cnki.syfjx.2011.15.038.

NIU Xiao-jing, SHI Jun-han. Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 90-92. DOI： 10.13422/j.cnki.syfjx.2011.15.038.

摘要

目的:建立同时测定安渴胶囊中马钱苷和葛根素含量的高效液相色谱法。方法:色谱柱为Aglient Eclipse XDB-C18柱

流动相乙腈-水（12∶88）

检测波长240

250 mm

流速1.0 mL.min-1。结果:马钱苷进样量在0.31～2.175μg与峰面积积分值呈良好的线性关系

葛根素进样量在0.164～1.148μg与峰面积积分值呈良好的线性关系。马钱苷平均回收率为100.39%

RSD 0.72%（n=6）;葛根素平均回收率为97.99%

RSD 1.98%（n=6）。结论:高效液相色谱法简便可行、专属性强、重复性好

可较好地控制安渴胶囊的质量。

Abstract

Objective:To establish the method for the assaying of loganin and puerain in Anke capsule with HPLC method.Method:The chromatographic column was Aglient Eclipse XDB-C18.The mobile phase was consisted of acetonitrile-water（12:88）.The detection wavelength was 240 nm and 250 mm.The flow rate was 1.0mL.min-1.The injection volume was 10 μL.Result:Loganin was linear in the range of 0.31-2.175 μg（r =0.999 9）.Puerarin was a linear in the range of 0.164-1.148 μg（r=0.999 9）.The average recovery of loganin was 100.39% and RSD was 0.72%（n=6）.The average recovery of puerain was 97.99% and RSD was 1.98%（n=6）.Conclusion:This method was simple and specificity with good repeatability

it can be used for the quality control of Anke capsule.

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