Objective:To find the effective components of Zhuanggu Zhitong prescription

investigate its influence on hormones related to bone metabolism and to explore its possible mechanism.Method:One hundred and fifty SD female rats weighing around 250 g（three months old） were randomly divided into model group（ovariectomized

OVX）

sham operation group

control group

ethanol extraction group A

ethanol extraction group B

petroleum ether extraction A position group

water extraction site group A

water extraction site group B

Fraction extracted with ethyl acetate group A

Zhuanggu Zhitong prescription group

a total of 10 groups（n=15 each）.Five days after stitches of the operation of taking out ovaries

different treatments were carried out for 13 weeks.Blood and bone samples were collected for investigations on bone mineral density

bone biomechanics

pathology and other aspects to evaluate efficacy

and serum estradiol（E2）

bone gla protein（BGP）

parathyroid hormone（PTH）

calcitionin（CT）

to explore the effective mechanism.Result:Compared with model group

Zhuanggu Zhitong prescription group A part of ethanol extraction

water extraction B parts

petroleum ether A site could significantly increase the levels of OVX rats femur

lumbar spine bone mineral density（P<0.05

P<0.01）;could increase osteoporosis in ovariectomized rat tibia model the maximum stress and maximum bending strength（P<0.05

P<0.01）;could significantly increase the average trabecular bone density of the femoral head

the difference was significant（P<0.01）

with no significant difference to Zhuanggu Zhitong prescription group.Compared to OVX model group

Zhuanggu Zhitong prescription could only significantly increase in serum E2

prevent uterine atrophy caused by insufficient estrogen（P<0.05）;Zhuanggu Zhitong prescription could significantly decrease serum BGP

PTH

CT for the castrated rats（P<0.05）.Conclusion:The ethanol extract parts of group A

water extraction site group B

petroleum ether extract A position group site are the effective components of Zhuanggu Zhitong prescription for treating osteoporosis.Zhuanggu Zhitong prescription can increase the serum level of E2

and decrease the levels of BGP

PTH

CT

directly or indirectly regulate the body’s bone metabolism and cytokines

to improve hormone metabolism

the molecular structure of bone matrix and to strengthen the mechanical properties of bone

therefore to treat osteoporosis.