天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响

李晓倩; 王兴; 李莹; 袁琳

doi:10.13422/j.cnki.syfjx.2011.15.051

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天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响

药理 | 更新时间：2024-09-23

- 天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响
- Impact of Compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on Renal Gene Expression of Kidney in SHR Rats
- 中国实验方剂学杂志 2011年17卷第15期页码：131-135
- 作者机构：
  
  西南交通大学生命科学与工程学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30801547）;中央高校基本科研业务会专项资金（SWJTU09ZT29）
- DOI：10.13422/j.cnki.syfjx.2011.15.051
  中图分类号： R285.5
- 纸质出版日期：2011
- 稿件说明：
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[1]李晓倩,王兴,李莹,袁琳.天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响[J].中国实验方剂学杂志,2011,17(15):131-135.

LI Xiao-qian, WANG Xing, LI Ying, et al. Impact of Compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on Renal Gene Expression of Kidney in SHR Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 131-135.
[1]李晓倩,王兴,李莹,袁琳.天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响[J].中国实验方剂学杂志,2011,17(15):131-135. DOI： 10.13422/j.cnki.syfjx.2011.15.051.

LI Xiao-qian, WANG Xing, LI Ying, et al. Impact of Compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on Renal Gene Expression of Kidney in SHR Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 131-135. DOI： 10.13422/j.cnki.syfjx.2011.15.051.

摘要

目的:研究天麻与钩藤配伍前后对SHR大鼠肾脏相关基因表达的影响。方法:设立空白、天麻混悬液（0.86 g.kg-1组）、钩藤煎液（1.17 g.kg-1组）、天麻钩藤合煎剂（天麻0.86 g.kg-1+钩藤1.17 g.kg-1）4个组别

连续ig给药10 d后提取肾脏mRNA

与基因芯片进行杂交

经扫描、计算机软件分析后观察配伍前后基因表达的变化。结果:筛选出天麻与钩藤配伍前后在肾脏中的目标基因（target gene）30个。结论:天麻配伍钩藤后

肾脏中调控血脂代谢、G-蛋白偶联受体介导的信号通路以及胰岛素抵抗相关基因的表达发生逆转

提示药对配伍后减弱了天麻对肾脏的相关作用。

Abstract

Objective:To study the impact of compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on renal gene expression in SHR rats.Method:Four SHR rat groups were established

including Blank

Gastrodia suspension 0.86 g·kg-1 group

Uncariae Remulus Cum Unicis decoction 1.17 g·kg-1 group

Gastrodiae Rhizoma 0.8 g·kg-1 and Uncariae Ramulus Cum Uncis 1.17 g·kg-1 decoction combined.After 10 days of continuous oral administration

mRNA in the kidney of SHR rats was extracted

and then was hybrided with gene chip.After computerized scanning and analyzing

the changes in gene expression were observed.Result:Thirty target genes before and after compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis were selected out.Conclution:After the compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis

the trends of gene expression related with lipid metabolism

G-protein coupled receptor mediated signaling pathway

and insulin resistance are reversed.It indicates that the related effects of Gastrodiae Rhizoma on kidney are weakened after the compatibility.

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