Objective:To study the protective effects of Modified Xionggui Decoction（MXD） against global cerebral ischemia-reperfusion injury（GCIRI） in rats.Method:According to Pulsinelli’s method

bilateral vertebral artery of rats was occlusion by electrocautery

followed by blocking blood flow of bilateral common carotid artery for 10 min

then reperfusion as GCIRI model.Ninety-six Sprague Dawley（SD） rats were randomly divided into 6 groups

one of them was sham-operation control group

and the others were GCIRI models.Three doses of MXD（0.38

0.76

1.52 g·kg-1） and Tongmai Granule（2.7 g·kg-1） were administered intragastrically respectively for 9 days

and water was used instead of drugs in Sham-operation and model control groups.The rats were pre-treatment of drug for 6 days before preparing GCIRI model and post-treatment for 3 days after establishment of GCIRI model.Neurological severity scores（NSSs） in the rats were observed 3 d after reperfusion.At the 4th day after reperfusion

the water content of the cerebal tissue was determined by weighing method

and concentration of malondialdehyde（MDA） were measured by TBA method in the plasma and the cerebal tissue

and activities of superoxide dismutase（SOD）

creatine kinase（CK） and lactate dehydrogenase（LDH） were assayed by spectrophotometry

and caspase-3 was tested by immunohistochemistry.Pathology of the brain were observed under microscope.Result:Compared with the model group

the brain water content

the NSS

the concentration of MDA and the expression of neurocyte caspase-3 in the pallium were degraded in MXD group.The activity of SOD

CK and LDH was increased in MXD group（P<0.05）.Conclusion:MXD has obvious protective effects against cerebal IR injury.