人参皂苷Rg<sub>3</sub>抑制腺样囊性癌细胞SACC-83增殖作用研究

苏涛; 孙宏晨; 马旭东

doi:10.13422/j.cnki.syfjx.2011.23.012

您当前的位置：

首页 >

文章列表页 >

人参皂苷Rg₃抑制腺样囊性癌细胞SACC-83增殖作用研究

药理 | 更新时间：2024-09-23

- 人参皂苷Rg₃抑制腺样囊性癌细胞SACC-83增殖作用研究
- Inhibition Effect of Ginsenoside Rg₃ on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83
- 中国实验方剂学杂志 2011年17卷第23期页码：195-198
- 作者机构：
  
  1. 湖州师范学院医学院
  2. 吉林大学口腔医学院病理科
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30672338）
- DOI：10.13422/j.cnki.syfjx.2011.23.012
  中图分类号： R285.5
- 纸质出版日期：2011
- 稿件说明：
移动端阅览
[1]苏涛,孙宏晨,马旭东.人参皂苷Rg_3抑制腺样囊性癌细胞SACC-83增殖作用研究[J].中国实验方剂学杂志,2011,17(23):195-198.

SU Tao1, SUN Hong-chen2, MA Xu-dong1. Inhibition Effect of Ginsenoside Rg₃ on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 195-198.
[1]苏涛,孙宏晨,马旭东.人参皂苷Rg_3抑制腺样囊性癌细胞SACC-83增殖作用研究[J].中国实验方剂学杂志,2011,17(23):195-198. DOI： 10.13422/j.cnki.syfjx.2011.23.012.

SU Tao1, SUN Hong-chen2, MA Xu-dong1. Inhibition Effect of Ginsenoside Rg₃ on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 195-198. DOI： 10.13422/j.cnki.syfjx.2011.23.012.

摘要

目的:探讨Rg3抑制人腺样囊性癌细胞的作用及其诱导凋亡的机制。方法:用一定质量浓度的Rg3处理人腺样囊性癌细胞系SACC-83细胞

MTT法检测细胞增殖活力

荧光显微镜下观察凋亡细胞的形态

流式细胞仪分析细胞周期和细胞增殖指数

免疫组织化学观察hTERT的表达变化。结果:Rg3抑制SACC-83细胞生长

呈时间-浓度效应关系;经20 mg.L-1 Rg3处理细胞24

48 h后

观察到典型的凋亡细胞形态

即染色质凝集、核片段化、凋亡小体、核内致密的颗粒状荧光;用不同浓度的Rg3处理细胞72 h后

均诱导了细胞凋亡和细胞周期的改变

G0/G1的细胞比率增加

S期和G2/M期的细胞比率下降

细胞增殖指数降低

并与Rg3浓度有明显依赖关系;免疫组化染色显示Rg3抑制了SACC-83细胞的hTERT蛋白表达。结论:Rg3可通过诱导人腺样囊性癌细胞SACC-83细胞凋亡和下调细胞的端粒酶活性

使细胞增殖受到抑制。

Abstract

Objective:To explore the effect of Rg3 on inhibiting and inducing apoptosis of human adenoid cystic carcinoma cells.Method: The human adenoid cystic carcinoma cell line SACC-83 was treated with Rg3 of various concentrations.Cell proliferation was measured by MTT assay.Morphological changes of cells were observed by fluorescent microscope.Cell cycle and cell proliferation index were analyzed by flow cytometry（FCM）.The expression of hTERT in cells was detected by immunohistochemistry.Result: Rg3 inhibited proliferation of SACC-83 cells in a manner of time-concentration-dependent relationship.When treated with 20 mg·L-1 of Rg3 for 24 h and 48 h

the cells showed apoptotic morphological characteristics including the condensed chromatin

the nuclear fragmentation

the apoptotic body and bright fluorescentgranules.When treated with Rg3 of various concentrations for 72 h

the percentage of cells in G0/G1 phase was increased

whereas the percentage of cells in S phase and G2/M phase was decreased.The cell proliferation index was decreased in a manner of obvious concentration-dependent relationship.The expression of hTERT in cells was inhibited by Rg3.Conclusion: The results suggest that ginsenoside Rg3 exerts an inhibiting effect on proliferation of SACC-83 cells by inducing apoptosis and down regulation of telomerase activity.

关键词

Keywords

references

浏览量

121

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

巴马汀结合MYC G-四链体干预结肠癌细胞增殖和凋亡的作用及分子机制

沙漠绢蒿黄酮抑制肺癌A-549细胞增殖

芍药汤含药血清介导Fas/FasL信号通路抑制脂多糖诱导的Caco-2细胞炎症及凋亡

黄芪甲苷通过SLC7A11/GPX4通路调控铁死亡抗血管平滑肌细胞增殖的作用机制分析

人参皂苷Rg3抑制腺样囊性癌细胞SACC-83增殖作用研究

Inhibition Effect of Ginsenoside Rg3 on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83

DOI：10.13422/j.cnki.syfjx.2011.23.012

摘要

Abstract

关键词

Keywords

references

人参皂苷Rg₃抑制腺样囊性癌细胞SACC-83增殖作用研究

Inhibition Effect of Ginsenoside Rg₃ on Proliferation of Human Adenoid Cystic Carcinoma Cell Line SACC-83