Objective:Established evaluation method of THTPM（traditional human taste panel method） which is suitable for bitterness study of traditional Chinese medicine decoction.Method: Choose single herb decoction of 35 different intensity of bitterness

with different concentrations of berberine hydrochloride solution as reference solution

using default of intensity of bitterness ratings and scoring table to evaluate taste test results of 20 healthy volunteers

removing outliers of the evaluation results by using Grubbs test method.Result:Got results of single herb decoction of 35 different bitterness intensition and distribution characteristics

and established for a system of THTPM which was suitable for bitterness study of traditional Chinese medicine decoction.Conclusion: Established method of THTPM in this paper was objectively and accurately and could be used as the reference method to evaluate bitterness of traditional Chinese medicine decoction.