Objective:To study the effects of Anshen Dingzhi Ling（ADL） on expression of dopamine transporter（DAT） of spontaneously hypertensive（SHR） rats’ striatum and the mechanism of this medicine to treat with ADHD.Method: Thirty SHR rats were randomly divided into 5 groups including model group

ritalin group

ADL high dose group

ADL medium dose group and ADL low dose group

6 rats each group

meantime

6 Wistar-Kyoto rats constitute normal control group.The dosages of the 3 groups of ADL were 34.1

17.1

8.5 g·kg-1

ig respectively and the dosage of ritalin group is 2.1 mg·kg-1

ig

as for the model group or normal control

rats were given physiological saline as per 10 mL·kg-1

ig.After 2-week

the rats in experiments were killed and separate the brain tissues

then examine the expression of striatum DAT protein or mRNA of each group with RT-PCR as well as laser scanning confocal microscope.Result: Statistic difference of DAT protein expression was observed among different groups（P<0.05）.Comparing with the normal control group

the DAT protein expression of striatum of SHR model group was higher obviously（P<0.01）;the DRT protein expression of ADL medium or high group was lower than model group（P<0.01）

meanwhile

the protein expression of ritalin group was lower than model group also（P<0.05）

but no statistic difference was observed between groups of low-dose ADL group and model in expression of DAT protein expression.The protein expression levels from the lower to the higher were ADL low dose group

ritalin group

ADL medium group as well as ADL high dose group.Conclusion: ADL has the potent to depress the expression of DAT mRNA as well as protein in SHR rat striatum

to block the reuptaking DA process performed by presynaptic neuron

and then to increase the DA concentration in synaptic clefts

to improve the conduction function of nerve signals of centrum DA

to play a role to treat with ADHD after all.