Objective:To observe the effects of Tianshengyuan oral solution（TSY）

a new candidate Chinese medicine

in mice with immunologically mediated aplastic anemia（IAA）.Method: Mouse models with IAA were developed and given TSY by gavage for 14 days.Then peripheral WBC

RBC

HGB

HCT

PLT

reticulocyte（RET）

femoral bone marrow nucleus cell（BMNC）

spleen index

and pathological features of femoral bone marrow and spleen tissues were examined.Result: The values of WBC

RBC

HGB

HCT

PLT

RET

BMNC

and spleen index in the model group were remarkably lower than those in the normal group（P

<

0.001）.TSY（12.4

24.8 g·kg-1·d-1）could significantly increase the amount of WBC（at the ratio of 150.0% and 133.3%

respectively）

RBC（9.5% and 37.1%）

HGB（12.9% and 44.5%）

HCT（11.6% and 28.4%）

PLT（94.8% and 252.3%）

RET（115.4% and 123.1%）

BMNC（71.1% and 95.6%）and spleen index（18.7% and 33.7%）

compared with IAA model group（P

<

0.05

0

.001）.Furthermore

pathological examination for the femoral bone marrow and spleen tissues indicated that TSY could obviously increase in hematopoietic cells

decrease in fatty cells

alleviate the atrophy of the splenic corpuscles

and accelerate the growth of hematopoietic focuses in spleen.Conclusion: TSY could promote the proliferation of hematopoietic cells inside and outside bone marrow

improve the function of hematopoiesis

thus have a great potential for the treatment of mice with IAA.