Objective:To establish a method for the determination about the contents of neomangiferin in the drug couple about Anemarrhenae Rhizoma and Gypsum Fibrosum

To explore the variation of the composition in the compatibility of different proportions.Method: High performance liquid chromatography（HPLC） was used to determine the changes in different groups of Anemarrhenae Rhizoma compatible with Gypsum Fibrosum.Separation was performed on C18（4.6 mm×250 mm

5 μm） analytical column.The mobile phase was ACN-0.2% CH3COOH（15∶85）.flow rate was 1.0 mL·min-1.detection wavelength was at 258.0 nm.Result:The contents of neomangiferin changed when Gypsum Fibrosum was added into the groups of Anemarrhenae Rhizoma.Some of ratios of Gypsum Fibrosum can increase the content of neomangiferin at first then decrease when the protortion was 3∶1（Gypsum Fibrosum-Anemarrhenae Rhizoma）.Conclusion:This method is simple

accurate and good repeatability.It can be used for the determination about the contents of neomangiferin in the drug couple about Anemarrhenae Rhizoma and Gypsum Fibrosum.The reseach proved the theory that ’the secret of Chinese medicine is due to the dose’

which points out the direction for further research.