ZHANG Ke-feng, CHEN Xu. Determination of Three Kinds of Flavonoid Aglycones in the Leaves of Camellia chrysantha(Hu)by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 60-62.
ZHANG Ke-feng, CHEN Xu. Determination of Three Kinds of Flavonoid Aglycones in the Leaves of Camellia chrysantha(Hu)by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 60-62. DOI: 10.13422/j.cnki.syfjx.2011.23.049.
金花茶叶中3种黄酮苷元的含量测定
摘要
目的:建立同时测定金花茶叶中槲皮素、木犀草素和山柰酚含量的高效液相色谱方法。方法:采用Agilent TC-C18色谱柱(4.6 mm×250 mm
Objective:Determination of three kinds of flavonoid aglycones in the leaves of Camellia chrysantha(Hu)by RP-HPLC.Method:The determination of three kinds of flavonoid aglycones was carried out with Agilent TC-C18 column(4.6 mm×250 mm
5 μm)
using methanol-water(52∶48)and flow rate of 1.0 mL·min-1and detected at the wave-length of 365 nm.Result: The quercetin and kaempferol of quercetin
luteolin and kaempferol were linear in the range of 3.3-16.7
2.7-28.8
4.1-14.7 mg·L-1 respectively.The average recovery was 98.00%
99.06%
98.11%.RSD were 1.27%
0.62%
0.94%.Conclusion:The methods were simple
rapid and reliable and could be used to control the quality of the C.chrysantha(Hu).