益肺活血颗粒对缺氧培养大鼠肺动脉平滑肌细胞NO,iNOS的影响

张凌云; 欧敏; 黄友章; 乔媛媛; 张达矜

doi:10.13422/j.cnki.syfjx.2011.23.057

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益肺活血颗粒对缺氧培养大鼠肺动脉平滑肌细胞NO,iNOS的影响

药理 | 更新时间：2024-09-23

- 益肺活血颗粒对缺氧培养大鼠肺动脉平滑肌细胞NO,iNOS的影响
- Effect of Yifei Huoxue Granule on Nitric Oxide and Inducible Nitric Oxide Synthesis in Rat Pulmonary Artery Smooth Muscle Cells Cultured in Chronic Hypoxia Condition in vitro
- 中国实验方剂学杂志 2011年17卷第23期页码：117-121
- 作者机构：
  
  1. 南方医科大学研究生学院
  2. 解放军海军总医院
- 作者简介：
- 基金信息：
  
  全军医药卫生科研基金项目（36040）
- DOI：10.13422/j.cnki.syfjx.2011.23.057
  中图分类号： R285.5
- 纸质出版日期：2011
- 稿件说明：
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[1]张凌云,欧敏,黄友章,乔媛媛,张达矜.益肺活血颗粒对缺氧培养大鼠肺动脉平滑肌细胞NO,iNOS的影响[J].中国实验方剂学杂志,2011,17(23):117-121.

ZHANG Ling-yun1, OU Min2, HUANG You-zhang2, et al. Effect of Yifei Huoxue Granule on Nitric Oxide and Inducible Nitric Oxide Synthesis in Rat Pulmonary Artery Smooth Muscle Cells Cultured in Chronic Hypoxia Condition in vitro[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 117-121.
[1]张凌云,欧敏,黄友章,乔媛媛,张达矜.益肺活血颗粒对缺氧培养大鼠肺动脉平滑肌细胞NO,iNOS的影响[J].中国实验方剂学杂志,2011,17(23):117-121. DOI： 10.13422/j.cnki.syfjx.2011.23.057.

ZHANG Ling-yun1, OU Min2, HUANG You-zhang2, et al. Effect of Yifei Huoxue Granule on Nitric Oxide and Inducible Nitric Oxide Synthesis in Rat Pulmonary Artery Smooth Muscle Cells Cultured in Chronic Hypoxia Condition in vitro[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 117-121. DOI： 10.13422/j.cnki.syfjx.2011.23.057.

摘要

目的:观察益肺活血颗粒对缺氧条件下大鼠肺动脉平滑肌细胞（PASMCs）一氧化氮（NO）产生及诱导型一氧化氮合酶（iNOS）活性的影响。方法:采用组织块贴壁法培养大鼠PASMCs

取对数生长期PASMCs随机分为常氧组

缺氧组

缺氧+益肺活血颗粒组（7.5

1.5

0.15 g.L-1）。利用硝酸还原酶法测定各组PASMCs NO的产生

RT-PCR测定iNOS mRNA的水平

免疫组化法测定胞内iNOS蛋白的表达量。结果:与单纯常氧组相比

缺氧组PASMCs iNOS转录和翻译增强

NO生成显著升高（P<0.05）;且与单纯缺氧组相比

益肺活血颗粒组随着药物浓度的增加

可进一步提高PASMCs iNOS的活性和NO的生成（P<0.05）。结论:益肺活血颗粒可以促进缺氧条件下PASMCs NO产生及iNOS活性和表达

其可能是通过提高iNOS基因的转录和蛋白的表达来实现

从而抑制PASMCs的增殖及介导血管的舒张

在缺氧性肺动脉高压方面有着独特的疗效。

Abstract

Objective:To investigate the effect of Yifei Huoxue granule（YFHXG） on the nitric oxide（NO） leveland inducible nitric oxide synthesis（iNOS） activation of rat pulmonary artery smooth muscle cells（PASMCs） upon exposure to chronic hypoxic conditions.Method: Tissue block anchorage was useed to the primary culture of rat PASMCs

PASMCs were randomly divided into normoxia group

hypoxia group

hypoxia+ YFHXG group（7.5

1.5

0.15 g·L-1）.NO level was measured by nitrate reductase method

mRNA abundance of iNOS was detected by RT-PCR

the expression of iNOS were examined by immunohistochemistry.Result: Compared with PASMCs in normoxia group

hypoxia caused NO levels to increase

the transcription and translation of iNOS improved a lot in hypoxia group（P<0.05）;But compared with hypoxia alone group

YFHXG group had a significantly effect in increasing NO level and iNOS activity which was based on hypoxia induced（P<0.05）.Conclusion: YFHXG can directly promote NO level and iNOS activation of PASMCs on hypoxic conditions through enhancing the transcription and translation of iNOS

thus inhibiting the proliferation of PASMCs and mediating vasodilation

it may play a crucial role in the protecting of hypoxic pulmonary arterial hypertension.

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