Objective:To observe the effect of ten traditional Chinese medicine and pseudolaric acid B（PB） on Candida albicans yeast and biofilm in vitro.Method:Moutan Cortex

Coptidis Rhizoma

Caryophylli Flos

Cinnamomi cortex

Alpiniae Officinarum Rhizoma

Cinnamomi Rhizoma Anemarrhenae Rhizoma

Sophore flavescentis Radir

Cnidii Fructus and Hedyotis diffusa Willd.were extracted with water

dissolved in DMSO.The microtiter method was used to test the antifungal effect against planktonic and biofilm C.albicans in vitro.Result: The MIC of PB inhibit the planktonic C.albicans was 15.6 mg·L-1

The MIC50 of PB inhibit the biofilm C.albicans was 31.2 mg·L-1.The MIC50 of Moutan Cortex and Alpiniae Officinarum Rhizoma inhibit the biofilm C.albicans was 125 mg·L-1 and 250 mg·L-1

respectively.The other medicines did not show any antifungal effect at 2 000 mg·L-1.Conclusion: PB showed some antifungal effect against planktonic and biofilm C.albicans

all the other traditional Chinese medicine water preparations did not show much activity against planktonic and biofilm C.albicans.The water extracts of traditional Chinese medicine did not showed much antifungal activity against the planktonic and biofilm C.albicans.The evaluation of the antifungal activity of traditional Chinese medicine need to be further discussed.