Objective:To choose the optimization conditions for the formation of total flavonoids in herba epimediumon complex with phospholipid.Method:Solvent evaporation process was used to prepare total flavonoids in herba epimediumon phospholipid complex.The evaluation criterion is complex ratio.The experiment inspect the significance of influential factor of preparation technology by Plackett-Burman design.Optimized significance of factor and level with the Box-Behnken design.The binomial and linear equations were fitted to the data of overall desirabilities

and the resulting equation was used to produce 3-D response surface graphs

through which optimal formulation were predicted.Result:Total flavonoids and phospholipid ratio

flavonoids concentration and reaction temperature had significance influence to the craft.It showed that the correlation coefficient of second-order quadratic model was high.Correlation coeficient reached 0.988.The optimum conditions for the preparation of total flavonoids in epimediumon complex were as follows:phospholipids-total flavonoids being 3.6∶1

time 2.4 h

temperature at 40 ℃ and concentration of principal agent being 10 g·L-1.The deviation between result of the best craft verification test and the binomial fitting equation forecast value is less than 2.0%.Conclusion:Response surface methodology was successfully used to optimize the preparation of total flavonoids in herba epimediumon phospholipid complex.Optimized process was reliable

stable and available for industrial production.