Objective:To predict the potential molecular target proteins of multiple constituents of Shenmai San absorbed in plasma based on computational systems biology approaches.Method:TCMGeneDIT was used to collected data regarding genes or proteins affected by Panax ginseng

Ophiopogon japonicus.and Schisandra chinensis.Based on the chemical structure of 14 main compounds absorbed into plasma after oral administration of Shenmai San

target identification and analysis were conducted to determine the target proteins of those compounds using PharmMapper sever.The proteins which have direct interaction with predictive target proteins were selected by screening BIND

ioGRID

DIP

HPRD

IntAct

MINT database.Result:Fifty five genes or proteins associated with P.ginseng were found.One gene or protein associated with S.chinensis was found.Four components of P.ginseng not only directly interact with target proteins which were proved by experiments

but also interact with other related proteins.Ten components of S.chinensis can only play assistant roles by interacting with related proteins.Conclusion:The result provided useful evidence for further research.It is expected that it be helpful for understanding the molecular mechanism of Shenmai San.Taken together

the protocol developed in this study may lead to a deeper and systematc understanding in the mechanism of Chinese formula.