LIU Bu-ming1, LIU Si-xiang1, LIN Xiao1, et al. Study on HPLC Chromatographic Fingerprint of the Mahonia Fortunei[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 95-99.
LIU Bu-ming1, LIU Si-xiang1, LIN Xiao1, et al. Study on HPLC Chromatographic Fingerprint of the Mahonia Fortunei[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 95-99. DOI: 10.13422/j.cnki.syfjx.2012.04.034.
十大功劳HPLC指纹图谱及聚类分析
摘要
目的:对十大功劳的HPLC指纹图谱及聚类分析进行研究
用以评价与控制药材内在质量。方法:采用HPLC法分析阔叶、长柱十大功劳的指纹图谱
运用指纹图谱相似度评价和聚类分析进行研究。结果:建立了十大功劳指纹图谱
确定10个色谱峰为共有峰
方法的精密度、稳定性、重现性良好
其指纹图谱相似性高
特征性和专属性强。结论:阔叶、长柱十大功劳的指纹图谱化学成分基本相同
但含量差异较大
相似度与聚类分析显示二者无明显差异
利用指纹图谱可对十大功劳进行质量控制。
Abstract
Objective:To establish the method of fingerprint analysis on Mahonia Forunei for controlling the quality.Method:The main components of the Mahonia Forunei from different sampes were determined by HPLC.Result:Ten common peaks were confirmed on the fingerprint
studying on the fingerprint of the Mahonia Fortunei.Conclusion:The method is reliable and accurate with good reproducibility and characteristic
providing a scientific base for controlling the quality of Mahonia Forunei by establishing fingerprint of the Mahonia Forunei.