ZHUANG Xiao-wei1, LIU Xiu-fang1, MAO Gui-yuan1, et al. Determination of Lethariella cladonioides Polysaccharide from Tibet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 103-106.
ZHUANG Xiao-wei1, LIU Xiu-fang1, MAO Gui-yuan1, et al. Determination of Lethariella cladonioides Polysaccharide from Tibet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 103-106. DOI: 10.13422/j.cnki.syfjx.2012.04.036.
Objective:To establish a method for the determination of polysaccharide in Lethariella cladonioides.Method:Using microplate reader
the content of total sugar was determined by means of phenol-sulfuric acid method and 3
5-dinitro salicylic acid(DNS) method was used for the determination of reducing sugar
the difference between the two was the polysaccharide content.Results:The total sugar standard curve equation was OD=0.051 4 C-0.014 6(r=0.999 2) and reducing sugar standard curve equation was OD =0.022 9C-0.086(r=0.999 9).Respectively the linear range was 2.5-12.5 mg.L-1 and 8-40 mg.L-1.Thepolysaccharide content of LC