HPLC-MS识别苦木中苦参碱和氧化苦参碱

刘丽娜; 孙磊; 程显隆; 田金改; 金红宇

doi:10.13422/j.cnki.syfjx.2012.04.038

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HPLC-MS识别苦木中苦参碱和氧化苦参碱

化学与分析 | 更新时间：2024-09-23

- HPLC-MS识别苦木中苦参碱和氧化苦参碱
- Identification of Matrine and Ammothamnine in Picrasmae Lignum by High Performance Liqid Chromatography /Tandem Mass spectromety
- 中国实验方剂学杂志 2012年18卷第4期页码：110-113
- 作者机构：
  
  中国食品药品检定研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.038
  中图分类号： R284.1
- 纸质出版日期：2012
- 稿件说明：
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[1]刘丽娜,孙磊,程显隆,田金改,金红宇.HPLC-MS识别苦木中苦参碱和氧化苦参碱[J].中国实验方剂学杂志,2012,18(04):110-113.

LIU Li-na, SUN Lei, CHENG Xian-long, et al. Identification of Matrine and Ammothamnine in Picrasmae Lignum by High Performance Liqid Chromatography /Tandem Mass spectromety[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 110-113.
[1]刘丽娜,孙磊,程显隆,田金改,金红宇.HPLC-MS识别苦木中苦参碱和氧化苦参碱[J].中国实验方剂学杂志,2012,18(04):110-113. DOI： 10.13422/j.cnki.syfjx.2012.04.038.

LIU Li-na, SUN Lei, CHENG Xian-long, et al. Identification of Matrine and Ammothamnine in Picrasmae Lignum by High Performance Liqid Chromatography /Tandem Mass spectromety[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 110-113. DOI： 10.13422/j.cnki.syfjx.2012.04.038.

摘要

目的:利用高效液相色谱-串联质谱联用分析和鉴定苦木中苦参碱及氧化苦参碱。方法:采用高效液相色谱-电喷雾/四极杆-飞行时间串联质谱

通过与对照品保留时间、准确分子量及其一级、二级质谱比对

进行成分鉴定

并建立了高效液相色谱-串联四级杆质谱联用测定苦木中苦参碱与氧化苦参碱的定量方法研究。结果:苦木中含有微量的苦参碱及氧化苦参碱成分

建立的方法专属、灵敏

可作为苦木中苦参碱及氧化苦参碱的识别依据。结论:首次以LC-MS鉴别出了苦木中苦参碱与氧化苦参碱。不同产地不同部位的苦木药材中苦参碱与氧化苦参碱含量差别较大。

Abstract

Objective:Identification of matrine and ammothamnine in picrasmae lignum by high performance liqid chromatography /Tandem Mass spectrometry.Method:Two alkaloids in picrasmae lignum were ananlyzed by HPLC-ESI /Q-TOF MS /MS.According to the accurate molecular weight

retention time and MSn data by compared with reference substances

the chemical structures were identified.After that

quantitative method was built based on the techniques of LC-Q-Q-Q.Result:Two trace components were identified in picrasmae lignum.The method is specific

sensitive and can be used as the identification of matrine and oxymatrine in picrasmae lignum.Conclusion:It was the first time to report the identification of Matrine and ammothamnine in picrasmae lignum by LC-MS.The content of matrine and ammothamnine in picrasmae lignum vary greatly with the different part and the area of produce.

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