Objective:To develop an effective HPLC method based on a reaction of phenylisothiocyanate（PITC） with glucosamine（GL） in alkaline media for the determination of glucosamine hydrochloride in dosage forms.Method:Reverse phase chromatography using pre-column derivatization with phenylisothiocyanate

and ultraviolet detection（254 nm） was used to quantify the eluate.The reaction produces a phenylthiocarbamyl-glucosamine（PTC-GL） adduct which was separated on a reverse-phase（RP） column packed with Agilent TC C18（4.6 mm×250 mm

5 μm）.The mobile phase consisted of pH 5.8 phosphate buffered saline acetonitrile（90︰10） and was pumped at a flow rate of 0.8 mL·min-1

and the column temperature was maintained at 30 ℃.Galactosamine hydrochloride（Gal-HCl） was used as an internal standard.Result:The standard curves for GL-HCl showed linearity（r=0.999 1） over the selected concentration range from 350 to 550 mg·L-1 for dosage forms.The average recoveriy of glucosamine hydrochloride was 98.3%（RSD 2.3%）.Conclusion:The method was found to be specific and with excellent linearity

accuracy and precision and is well suited for the quantitation of GL-HCl in dosage forms.