LIU Yu-qiang, ZHEN Bi-xian, CAI Qian. Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 120-122.
LIU Yu-qiang, ZHEN Bi-xian, CAI Qian. Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 120-122. DOI: 10.13422/j.cnki.syfjx.2012.04.041.
不同地区白术药材及饮片中白术内酯Ⅱ的含量测定
摘要
目的:采用HPLC测定不同地区白术药材及饮片中白术内酯Ⅱ的含量。方法:采用Tigerkin C18(4.6 mm×200 mm
Objective:To determine the content of atractylenolide Ⅱ in crude drugs and slices of Atractylodes macrocephala from different areas by HPLC.Method:The samples were separated on Tigerkin C18column(4.6 mm×200 mm
5 μm)with the mobile phase of methanol-water(77∶23).Flow rate was 1.0 mL·min-1.The detection wavelength was set at 276 nm.Column temperature was maintained at 30 ℃.Result:The contents of atractylenolide Ⅱ in crude drugs from 10 places of origin and slices and processing slices of A.macrocephala from 20 regions was determined.Conclusion:The contents of atractylenolide Ⅱ in crude drugs and slices of A.macrocephala from different regions was different.