LI Ying1, HUANG Yan-fei1, LIU Yuan1, et al. Studies on Fingerprint and Identification of Main Species of Acanthopanax in Tibetan and Qiang Ethnic Area of Sichuan[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 142-145.
LI Ying1, HUANG Yan-fei1, LIU Yuan1, et al. Studies on Fingerprint and Identification of Main Species of Acanthopanax in Tibetan and Qiang Ethnic Area of Sichuan[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 142-145. DOI: 10.13422/j.cnki.syfjx.2012.04.048.
Objective:To establish an effective method for identification of main species of Acanthopanax in Tibetan and Qiang Ethnic area of Sichuan by HPLC.Method:The analysis was carried out at 30 ℃ on a Kromasil C18 column eluted with a mobile phase consisted of methanol-0.5% phosphoric acid
and the data were analyzed with‘Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica’software.Result:The established chromatographic fingerprint method was repeatability
among which 12 fingerprint peaks in common were confirmed.The DPSv3.0 statistics software was used for clustering analysis with the clustering method of Betweengroups linkage and Euclidean distance.The analysis result was that S1
S2 and S3 of the different medical part of A.giraldii Harms were mostly similar
then that of S5
S4
S8
S6 and S7 in turn.Conclusion:This chromatographic fingerprint could be used specifically to distinguish the main species of Acanthopanax Miq.in Tibetan and Qiang Ethnic area of Sichuan.