Objective:To study the hepatotoxicity of Farfarae Flos.Method:KM mice were given the water extracts of Farfarae Flos orally in 20

40 g·kg-1 doses for successive 4 weeks

enzyme activity of aspartate transaminase（AST）

alanine transaminase（ALT） in serum of mice were measured

coefficient of liver were calculated

and liver histopathology examination were conducted.Using the precision-cut slice technology

culture liver slices for 24 hours with water extracts in concentration 0.005

0.05 g·L-1 and 6 hours with the total alkaloid of Farfarae Flos in concentration 0.5

2.0 g·L-1

the slice homogenate were prepared

protein concentration were detected by BCA protein assay method

enzyme activity of ALT

AST

lactate dehydrogenase（LDH）

r-glutamgl thanspeplidase（GGT） were detected by enzyme kinetics method and leakage were calculated.Result:The water extract of Farfarae Flos 40 g·kg-1 dose group of female had significant histopathology changes and organ coefficient of liver increased compared with control group.After co-culture for 24 hours with water extracts of Farfarae Flos

ALT leakage were significantly increased in two groups;LDH and ALT leakage were significantly increased with final concentration 0.5 g·L-1

GGT leakage was significantly increased and protein content was obviously decreased with final concentration 2.0 g·L-1 after co-culture for 6 hours with the total alkaloid of Farfarae Flos.Conclusion:Farfarae Flos displayed liver toxicity in some degree for in vivo and in vitro experiments.