Objective:To investigate percutaneous penetration effect of different concentrations of volatile oil from Fructus Litseae（FL）

Rhizoma Zingiberis（RZ） and Flos Magnoliae（FM） on model drug-tetramethylpyrazine phosphate（TP）.Method: Modified Franz diffusion cell method was used

enhancing penetration effect through mouse skin of 1%

3%

5%

7% volatile oil extracted from FL

RZ and FM on LP were investigated with azone as comparison

the content of LP was determined by HPLC.Result: 5% azone

1% volatile oil from FL

3% volatile oil from RZ

5% volatile oil from FM had the best effect on enhancing permeation effect of LP through skin of mice

average cumulative permeation quantity on 20 g·L-1LP in 12 h（Q12）were 0.841 2

0.915 9

0.740 3

0.932 8 mg·cm-2·h-1

respectively;enhancing times in 4 h of each were 1.357

1.279

1.054

1.156

respectively.After t-test

Q12 of LP which added 1% volatile oil from FL was higher than the negative control group significantly.Conclusion: 1% volatile oil from FL

3% volatile oil from RZ and 5% volatile oil from FM could increase percutaneous permeation quantity of LP.