穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺

涂瑶生; 付建武; 孙冬梅; 张建军; 江志强; 李绍林; 谢菲

doi:10.13422/j.cnki.syfjx.2012.08.033

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穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺

工艺与制剂 | 更新时间：2024-09-23

- 穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺
- Purification Technology of Andrographolide and Dehydroandrographolide by HPD100
- 中国实验方剂学杂志 2012年18卷第8期页码：22-25
- 作者机构：
  
  1. 广东省中医研究所
  2. 广州中医药大学
  3. 广东一方制药有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.033
  中图分类号： R286.0
- 纸质出版日期：2012
- 稿件说明：
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[1]涂瑶生,付建武,孙冬梅,张建军,江志强,李绍林,谢菲.穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺[J].中国实验方剂学杂志,2012,18(08):22-25.

TU Yao-sheng1, FU Jian-wu1, SUN Dong-mei1, et al. Purification Technology of Andrographolide and Dehydroandrographolide by HPD100[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 22-25.
[1]涂瑶生,付建武,孙冬梅,张建军,江志强,李绍林,谢菲.穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺[J].中国实验方剂学杂志,2012,18(08):22-25. DOI： 10.13422/j.cnki.syfjx.2012.08.033.

TU Yao-sheng1, FU Jian-wu1, SUN Dong-mei1, et al. Purification Technology of Andrographolide and Dehydroandrographolide by HPD100[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 22-25. DOI： 10.13422/j.cnki.syfjx.2012.08.033.

摘要

目的:研究穿心莲内酯和脱水穿心莲内酯的大孔树脂吸附分离纯化工艺。方法:考察HPD100型大孔树脂的吸附性能和洗脱参数

采用HPLC测定穿心莲内酯和脱水穿心莲内酯含量。结果:HPD100型大孔树脂适宜的工艺条件为最大上样量为每克药材1.0 g树脂

5 BV 20%乙醇除杂

10 BV 35%乙醇

5 BV 55%乙醇溶液分别洗脱

总内酯纯度68.46%;35%乙醇洗脱液中主要富含穿心莲内酯;55%乙醇洗脱液中富含脱水穿心莲内酯。结论:HPD100型大孔吸附树脂在所确定的工艺条件下

对穿心莲内酯和脱水穿心莲内酯的纯化效果良好

对大生产有指导意义。

Abstract

Objective:To study on separation and purification technology of andrographolide and dehydroandrographolide by macroporous resin.Method: Based on screening model of resin

characteristics of adsorbing and eluting parameter had been obtained by HPD100 resin

and the content of andrographolide and dehydroandrographolide were determined by HPLC.Result: Optimum parameter of purification were as follows: maximum sample volume was 1.0 g of resin per medicine material

5 BV 20% ethanol was used for eluting impurity

for eluting target components was 10 BV 35% ethanol（for andrographolide） and 5 BV 55% ethanol（for dehydroandrographolide）.Purity of andrographolide and dehydroandrographolide were respectively 48.59% and 19.87% and total lactone was 68.46%.Conclusion: HPD100 resin was favorable to andrographolide and dehydroandrographolide under condition of determined process

and it had significance for large-scale production.

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