Objective:To study the dissolubility proteins and activity of fibrinolytic enzyme of fresh and dry earthworm comparably.Method: Bradfold method was used to detect protein content

SDS-PAGE was used to analyze the protein compositions

and the agarose-fibrin plate was used to detect the activity of fibrinolytic enzyme.Result: On dried basis

the protein extraction rate of fresh earthworm was（13.89±0.86）%

the protein content was（63.14±0.38）%

the activity of enzyme led to（8 743±17） IU·g-1;compared with dry earthworm

the protein extraction rate was（9.82±0.75）%

the protein content and enzyme activity were（45.16±0.12）% and（206±14） IU·g-1.The SDS-PAGE result showed that there were 12 protein bands in fresh earthworm and only several bands in dry earthworm.Conclusion: The extraction rate

content and enzyme activity of the dissolubility proteins at fresh earthworm were higher than dry earthworm

and the constituents exhibited obvious difference.