Objective:To find a quality evaluation method of Concisum Rhizoma Wenyujin.Method: HPLC fingerprint profiles of sixteen samples were established.Column Kromasil 100-5C18（4.6 mm×250 mm

5 μm） was used.The mobile phase was acetonitrile and water

and the flow rate was 0.8 mL·min-1.A matrix including 41 variations（characteristic peaks area） and 16 cases（samples） was constructed for similarity evaluation

cluster analysis and principle component analysis.Result: The results showed that the collected samples had a good similarity.A specificity fingerprint chromatogram was produced and fourteen characteristic peaks were designated.Sixteen samples were divided into three groups.Conclusion: It was a reliable and available method of quality control of Rhizoma wenyujin concisum and it was necessary for the safe and practicable use of this herbal medicine.