Objective:To probe rhynchophylline contents in part of Uncaria Schreb.plant breeds

and further provide experimental basis for evaluating the quality of medicinal materials.Method:Rhynchophylline in Uncaria Sandens

U.rhynchophylloides

U.hirsuta

U.lancifolia

U.homomalla and U.sinensis from 33 different producing areas were determined by HPLC.HPLC column was Gemini C18（4.6 mm×250 mm

5 μm）

mobile phase was methanol-0.002 mol·L-1 triethylomine（pH value 7.5

adjusted with acetic acid）（64∶36）

flow rate was 1.0 mL·min-1

and detection wavelength was 254 nm.Result: 5 batches of sample in U.Sandens and U.rhynchophylloides contained rhynchophylline

but content of rhynchophylline was low.The others do not contain rhynchophylline.Conclusion: The different breeds of Uncaria Schreb.plant vary in quality

while the quality of the same breed varies in different producing area.