Objective:To investigate the time-dependent metabolic profiles of hydrocortisone-induced Kidney-yang deficiency syndrome in rats and to identify the corresponding potential biomarkers.Method:Proton nuclear magnetic resonance（1H-NMR） technique was used to analyze the rat metabonome in urine.Principal components analysis（PCA） and orthogonal partial least squares discriminant analysis（OPLS-DA） were processed to analyze the time trajectories of urinary metabonome in rats with ’Kidney-yang deficiency syndrome’.OPLS-DA was employed to study the metabonome difference between the control and hydrocortisone treated samples.Potential biomarkers were selected via the parameter of variable importance in the projection（VIP）.Result:Time trajectories of urinary metabonome of hydrocortisone-treated rats were drawn.Thirteen potential biomarkers were selected in rat urine.Conclusion:After hydrocortisone treatment

the energy metabolism and gut microflora environment were seriously disturbed

and transmethylation and transportation of amine were surpressed

in combination with kidney impairment.The results obtained suggested that metabonomic studies could be treated as a potential powerful approach in investigation of the onset and progression of ’Kidney-yang deficiency syndrome’

the pathological mechanism studies

objective diagnosis and the evaluation of traditional Chinese medicines that can replenish the kidney.