ZHU Dan1, ZHANG Chun-hua2, LIANG Qiu-yun1, et al. Influences of Jinhuang Fuzheng Tea on Cellular Immune Function in Immunosuppressed Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 242-245.
ZHU Dan1, ZHANG Chun-hua2, LIANG Qiu-yun1, et al. Influences of Jinhuang Fuzheng Tea on Cellular Immune Function in Immunosuppressed Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 242-245. DOI: 10.13422/j.cnki.syfjx.2012.08.082.
金黄扶正茶对免疫抑制小鼠细胞免疫功能的影响
摘要
目的:探讨金黄扶正茶对免疫抑制小鼠脾脏T、B淋巴细胞增殖及腹腔巨噬细胞免疫功能的影响。方法:金黄扶正茶高、中、低剂量组分别连续给昆明种小鼠ig 10 d
Objective:To observe the effects of Jinhuang Fuzheng tea on spleen T
B lymphocyte proliferation and peritoneal macrophage immune function in the Immunosuppressed mice.Method: Mice were ig administrated Jinhuang fuzheng tea at high
middle and low dose for 10 days.The immunosuppression mouse model was caused by subcutaneous injection of cyclophosphamide(30 mg·kg-1) every other day.The determination of concanavalin A(Con A) induced T lymphocyte proliferation and lipopolysaccharide(LPS) induced B lymphocyte proliferation and natural killer cells(NK cells) and lymphokine-activated killer cells(LAK cells) activity was examined by tetrazolium blue(MTT).The inducible nitric oxide synthase(iNOS) secreted by peritoneal macrophages activity was measured by enzymic method.Automatic detection microplate reader was used to determine the capability of phagocytosis neutral red.Result: The immunosuppressed mice which were given Jinhuang Fuzheng tea at low
medium and high dose groups showed a increased T and B lymphocyte A value(P<0.01);The medium and high dose groups significantly improved NK cells LAK cell activity
phagocytic activity of peritoneal macrophages and iNOS activity in immunosuppressive mice(P<0.01
P<0.05).Conclusion: The Jinhuang Fuzheng tea can promote the immune function in Immunosuppressed mice.