Objective: To explore the protective effect of cerebrospinal fluid（CSF） with the optimized Danggui Shaoyao San（FBD） and its underlying mechanisms.Method: PC12 cells were treated with H2O2 / Fe2+ to reproduce the cell injury model.The rabbits were randomly divided into 1 d group and 14 d group and they were given FBD by intragastric administrate.PC12 cells were treated with different concentrations of CSF containing FBD（5%

10%

20%）.Cell survival was assessed by Thiazzolyl blue（MTT） assay.The content of maleic diadehyde（MDA） and superoxide dismutase（SOD） activity were determined respectively by thiobarbituric acid and xanthine oxidation enzyme.The Ca2+content within cell was measured by MTB colorimetry.The protein expression of α7 nicotinic acetylcholine receptor（nAchR） was examined by using immunocytochemical detecting kit

bicinchoninic acid（BCA） protein kit and Dot blot assay.Result: The 20% group for 1 day（1.241±0.117） and 14 days（1.297±0.213） of FBD improved the survival of the PC12.The content of SOD increased from（18.18±0.12） U·mL-1 in the 20% group for 1 d to（19.48±0.34）U·mL-1 and 14 d（19.52±0.33） U·mL-1（P<0.01）.In addition

α7nAChR in PC12 cells was significantly up-regulated.The expression of α7nAChR in 20% group for 14 d（0.161±0.024） was significantly higher than that in the group for 1 d（0.132±0.021）（P<0.05）.These groups reduced MDA and Ca2+within cell from（3.26±0.13）mmol·L-1

（74.89±3.66）nmol/105 cell and（2.77±0.05）mmol·L-1

（74.25±2.64）nmol/105 cell to（4.41±0.23）mmol·L-1

（82.42±4.25） nmol/105 cells).Conclusion: The CSF containing FBD can prevent from the neurotoxicity induced by H2O2 / Fe2+.It may be related to α7nAchR up-regulation.