Objective:To observe the effect of Sanhua Tang on expression of zona occludens-1（ZO-1） in brain tissues of cerebral ischemia-reperfusion rat.Method:The rats were randomly divided into sham operation group

model group

low dose group of Sanhua Tang

large dose group of Sanhua Tang and nimodipine group.The middle cerebral artery was blocked with suture method to prepare cerebral ischemia reperfusion model.After cerebral ischemia for two hours and reperfusion for twenty-fourth hours

the expression of ZO-1 was detected in every brain tissues by immunohistochemical technique.Result: Compared with sham operation group

in model group expression of ZO-1 in brain tissues of cerebral ischemia-reperfusion rat was significantly decreased（P<0.01）;compared with model group

expression of zo-1 in brain tissues was significantly increased in large dose group of Sanhua Tang（P<0.01）.In nimodipine group the expression of zo-1 was evidently increased（P<0.05）.there were no significant differences between model group and low dose of Sanhua Tang.Compared with nimodipine group

the expression of ZO-1 in brain tissues of cerebral ischemia-reperfusion rat was significantly increased in large dose group of Sanhua Tang（P<0.05）.Conclusion:Sanhua Tang can protect against injure of cerebral ischemia-reperfusion in rat.