ZHANG Fang1, ZHANG Yong-qing1, YU Xiao2, et al. Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 83-86.
ZHANG Fang1, ZHANG Yong-qing1, YU Xiao2, et al. Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 83-86. DOI: 10.13422/j.cnki.syfjx.2012.09.072.
Objective: To analyze and identify the buds of different germplasm resources of Lonicera japonica Thunb.cultivated in ShanDong.Method: Multi-steps infrared macro-fingerprint method(infrared spectroscopy
secondary derivative spectroscopy and two dimensional correlation infrared spectroscopy) was used to analyze the buds of different germplasm resources of Lonicera japonica Thunb.Result: In the one-dimensional spectra
there have some similarity among different germplasm
appearing the absorption peak of-OH in 3 371 cm-1
the absorption peak of-CH2 in 2 924 cm-1 and 2 853 cm-1
the absorption peak of C=O in 1 735 cm-1
the absorption peak of =C-O in 1 637 cm-1 and the absorption peak of C-O in 1 637 cm-1.In the second derivative spectra
there have significant difference among different germplasm between 1 800 cm-1 and 1 300 cm-1.Otherwise
the 2D-IR correlation spectra also have significant differences in the absorption strength
frequency and form.Conclusion: The FTIR Analysis is effective for the identification of the buds of different germplasm resource.