Objective: To study the HPLC fingerprint of Saxifraga stolonifera from Guizhou.Method: HPLC analysis was performed on a DiamonsiL C18 column（4.6 mm×250 mm

5 μm） with the gradient eluting system consisted of methanol and 0.1%H3PO4.The flow rate was 1.0 mL·min-1.The column temperature was maintained at 35 ℃ and the detection wavelength was at 250 nm.Result: There were twelve characteristic peaks indicated in fingerprints.The main composition of different origin S.stolonifera is basically the same.But there is a large difference between each content of components.Conclusion: This method can be used to determine fingerprint of S.stolonifera in different origin from Guizhou.And it helps to regulate the quality of medical resources and provide scientific basis in the future.