Objective:To observe the antibacterial and antiviral activities

and provide certain experimental data for strictosamide that is intended for upper respiratory tract infection.Method: The in vitro antibacterial activities on standard staphylococcus aureus and other 9 strains were observed by both test-tube and plating methods

and the protective effects on mice infected by staphylococcus aureus and streptococcus pneumonia were also observed.Mice were divided into 5 groups

such as normal group

model group

reduning group（crude drugs 8.7 g·kg-1）

low and high dosage（20

40 mg·kg-1） of strictosamide groups.They were injected intraperitoneally once a day for 7 days.At 1 h after the last injection

staphylococcus aureus or streptococcus pneumonia was injected intraperitoneally to mice

and then the death of mice was observed within 7 days.The in vitro antiviral activities were observed on the model of MDCK cells infected by influenza A（A/PR8/34（H1N1）） and B（B/Jingfang 98-76） virus

and the in vivo antiviral activities were observed by the model of mice infected by influenza A virus.Mice were divided into 5 groups

such as normal group

model group

reduning group（crude drugs 8.7 g·kg-1）

low and high dosage（20

40 mg·kg-1） of strictosamide groups.They were injected intraperitoneally once a day for 5 days

and virus was given to mice by nose on the first day.After the last injection

the lung lesions and pathological changes were observed

and the pulmonary index was also calculated.Result: Strictosamide had inhibiting effects on standard escherichia coli and other 6 strains in vitro with MIC of 5 g·L-1.At 10 g·L-1

the diameters of inhibiting bacteria circle of diplococcus pneumonia and other 4 strains were 13-25 mm.At 40 mg·kg-1

the survival rate of mice infected by staphylococcus aureus and streptococcus pneumonia was 70% and 55%

separately.Strictosamide showed inhibitory effects on both influenza A and B viruses in vitro with IC50 values of 0.649

0.323 g·L-1

respectively.It also significantly inhibited increased pulmonary index and improved pulmonary pathological lesions of the infected mice caused by influenza A virus

and the inhibitory rate of pulmonary index was 39.8% at 40 mg·kg-1.Conclusion: Strictosamide possesses antibacterial and antiviral activities.