鳖甲煎丸对免疫性肝纤维化大鼠肝组织HGF表达的影响

李志毅; 崔莉芳; 张伶俐; 程传浩; 谢世平

doi:10.13422/j.cnki.syfjx.2012.15.060

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鳖甲煎丸对免疫性肝纤维化大鼠肝组织HGF表达的影响

药理 | 更新时间：2024-09-23

- 鳖甲煎丸对免疫性肝纤维化大鼠肝组织HGF表达的影响
- Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis
- 中国实验方剂学杂志 2012年18卷第15期页码：192-195
- 作者机构：
  
  1. 河南中医学院
  2. 河南省中医药研究院
  3. 郑州市卫生学校
- 作者简介：
- 基金信息：
  
  河南省科技攻关项目（2000360016）
- DOI：10.13422/j.cnki.syfjx.2012.15.060
  中图分类号： R285.5
- 纸质出版日期：2012
- 稿件说明：
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[1]李志毅,崔莉芳,张伶俐,程传浩,谢世平.鳖甲煎丸对免疫性肝纤维化大鼠肝组织HGF表达的影响[J].中国实验方剂学杂志,2012,18(15):192-195.

LI Zhi-yi1, CUI Li-fang2, ZHANG Ling-li3, et al. Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 192-195.
[1]李志毅,崔莉芳,张伶俐,程传浩,谢世平.鳖甲煎丸对免疫性肝纤维化大鼠肝组织HGF表达的影响[J].中国实验方剂学杂志,2012,18(15):192-195. DOI： 10.13422/j.cnki.syfjx.2012.15.060.

LI Zhi-yi1, CUI Li-fang2, ZHANG Ling-li3, et al. Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 192-195. DOI： 10.13422/j.cnki.syfjx.2012.15.060.

摘要

目的:观察鳖甲煎丸对免疫性肝纤维化模型大鼠肝组织肝细胞生长因子（HGF）表达的影响

分析鳖甲煎丸抗肝纤维化的治疗作用并探讨其作用机制。方法:雄性Wistar大鼠90只

随机分为6组:正常对照组、模型组、秋水仙碱预防组、秋水仙碱治疗组、鳖甲煎预防组、鳖甲煎治疗组

采用猪血清诱导免疫损伤性肝纤维化模型。各组于10周后随机取8只大鼠断头处死

取血清

检测血清透明质酸（HA）、层黏连蛋白（LN）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（Ⅳ-C）;取肝组织

免疫组化染色

用图像分析法检测HGF。结果:各预防组、治疗组血清HA

PCⅢ

Ⅳ-C含量均明显降低（P<0.01或P<0.05）

但以鳖甲煎预防组、治疗组降低最为明显;各预防组、治疗组大鼠肝脏组织HGF均有表达（P<0.01或P<0.05）

鳖甲煎预防组、治疗组比秋水仙碱组的表达更为明显。结论:鳖甲煎丸能够抑制肝脏纤维化病理改变

增加HGF的表达

具有明显的抗肝纤维化作用。

Abstract

Objective: To observe the effects of Biejiajian Wan（BJJW） on hepatocyte growth factor（HGF） expression in the rats with immune hepatic fibrosis

and analyze therapeutic functions of anti-hepatic fibrosis of BJJW and explore the mechanism.Method: Ninety male Wistar rats were randomly divided into six groups: normal control group

model control group

colchicine prevention group

colchicine treatment group

BJJW prevention group

BJJW treatment group.The rat liver fibrosis model was produced by porcine serum.After 10 weeks eight rats from each group were selected randomly.The hepatic tissues of the rats were taken and stained by immunohistochemistry

HGF of them was detected by image analysis.Hyaluronic acid（HA）

laminin（LN）

collagen type Ⅲ（PCⅢ）

type Ⅳ collagen（Ⅳ-C）in serum.Result: Compared with model group

the level of HA

PCⅢ

Ⅳ-C was obviously decreased in preventive groups and therapeutic groups

but BJJW preventive groups and therapeutic groups decreased most significantly.The expression of HGF were obviously increased in preventive groups and therapeutic groups

but BJJW preventive and therapeutic groups increased most significantly.Conclusion: BJJW can inhibit the rat’s pathological changes of hepatic fibrosis

significantly increase the expression of HGF

which indicates the obvious function of anti-hepatic fibrosis.

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