Objective: To reveal the mechanism of drug action to compare and analyze of the effect of anti-tuberculosis particles extract on multi-drug resistant-mycobacterium tuberculosis proteomics to reveal the mechanism of drug action.Method:The clinical mycobacterium tuberculosis with multidrug resistance was added in Middlebrook 7H9 OADC liquid medium at 37 ℃ for 6-8 d

when the bacterial density researched 1×108/mL-2×108/mL

the minimum inhibitory concentration（MIC） of anti-tuberculosis particulate extracts was 0.638 8 g·L-1

the final concentration was equivalent to 0.255 6 g·mL-1.Local environment and external environment was completely isolated

two-dimensional gel electrophoresis separation of whole cell total protein was used to analyze differentially expressed protein spots.Result: Before and after treatment by anti-tuberculosis particles extract

multidrug-resistant mycobacterium tuberculosis showed eight differences spots

including two determined proteins: thiosulfate sulfur transferase and hypothetical protein Rv0634A.Conclusion: The results indicate that anti-tuberculosis particles have an impact on the translation

composition

amino acid metabolism

cyanide

H2S detoxification as well as sulfur and iron transfer of the multi-drug resistant mycobacterium tuberculosis

inhibition of mycobacterium tuberculosis may be contributed to undermining the livelihood of the mycobacterium tuberculosis ability.