Objective: Using in vitro and in vivo models

the role of bitter melon extract on protein non-enzymatic glycation were investigated.Method: In vitro model

different doses of the extract（0.01

0.1

1.0 g·L-1） or aminogunidine（4 mmol·L-1） were added

non-enzymatic glycation end products were measured at different time points by fluorescent measurer.In vivo model

diabetes mice were fed with different doses of extract（10

30 g·kg-1）.The effects of bitter melon extract on blood glucose

advanced glycation end products（AGEs） levels

superoxide dismutases（SOD） activity and maleic dialdehyde（MDA） content in heart

liver and kidney tissue were assayed.Result: In vitro system

protein glycation products formation was positively correlated with the incubation time

bitter melon extract inhibited the formation of advanced glycation end products dose-dependently（P<0.01）.Animal experiments showed that bitter melon extract inhibited blood sugar of diabetic mice（P<0.05

P<0.01）.AGE levels and MDA content of heart

liver and kidney tissue were lowed significantly in group which fed with extract（P<0.05

P<0.01）.Also

SOD activity was enhanced in group which fed with extract（P<0.05

P<0.01）.Conclusion: Bitter melon extract have inhibitive effects on the formation of advanced glycation end products in vitro and in vivo.